HLA-B*27 Real-time PCR Technical Information

HISTO TYPE B*27 Real-time PCR

Technical information


Product description

The HISTO TYPE B*27 quantitative real-time PCR kit is used for the molecular genetic detection of HLA-B*27 alleles. The HLA-B*27 protein is a variant of the human leucocyte antigen-B (HLA-B) and is associated with different autoimmune diseases (Morbus Bechterew or Ankylosing Spondylitis, Reiter’s disease, reactive arthritis) and is, therefore, used as part of the diagnostic procedure.

A positive HLA-B*27 result is associated with a high disease risk. In suspected cases of Ankylosing Spondylitis, a HLA-B*27 diagnosis provides an important contribution to the therapy of the patient. Around 3% to 6% of the people carrying the HLA-B*27 gene develop Ankylosing Spondylitis and more than 90% of all patients with a seronegative arthritis are carrying this gene. The HISTO TYPE B*27 Q kit covers all common HLA-B*27 subtypes. Moreover, the kit differentiates between the disease associated alleles and the subtypes HLA-B*27:06 or HLAB*27:09, which are not associated with Ankylosing Spondylitis.


Test principle

Genomic DNA is amplified in a PCR with sequence-specific primers (SSP). Amplicons are detected with gene locus specific fluorescent dye-labelled hydrolysis probes (TaqMan® probes), which increases the diagnostic sensitivity and specificity of the test compared to a conventional SSP. If amplicons are present, the probes are hydrolyzed and a fluorescence signal is generated which increases proportionally to the amount of the PCR product. The fluorescence signals are measured by the optical detection unit of the RT-PCR-Cycler. The test is performed in a single PCR reaction that detects the internal positive control (human HBB gene) the disease-associated subtypes and the non-disease-associated subtypes with different fluorescent colours.

  •  • Discrimination of the non-associated alleles (B*27:06 and B*27:09 for Morbus Bechterew/Ankylosing Spondylitis).
  •  • Includes all common and well documented B*27 alleles covered in one PCR mix.
  •  • Internal amplification control included.


    Instructions for Use- 

    HISTO TYPE B*27 Q Tests 


    To discuss your purchase requirements in complete confidence, please Contact us using our secure enquiry form, and one of our management team will get back in touch.

    Click any of the following links for further product information:

    why choose us?


    MC Diagnostics is a specialist molecular diagnostics company with expertise in the automation of multiplexed molecular assays and custom designed image analysis and interpretation software. Read more about our capabilities below ...

    Products...    Services...     Manufacturing...    Technology...

    contact us

    Please complete the secure form below to get in touch. If you prefer please use our SECURE Contact page.


      Call us now on:

    +44 (0) 1745 535225

     Address Location

    MC Diagnostics Ltd
    Unit 5 OpTIC
    Ffordd William Morgan
    St. Asaph Business Park
    LL17 OJD
    United Kingdom

    View Map

    Connect with us