HLA-B*27 Real-time PCR Technical Information

HISTO TYPE B*27 Real-time PCR
 


Technical information

 

Product description

The HISTO TYPE B*27 quantitative real-time PCR kit is used for the molecular genetic detection of HLA-B*27 alleles. The HLA-B*27 protein is a variant of the human leucocyte antigen-B (HLA-B) and is associated with different autoimmune diseases (Morbus Bechterew or Ankylosing Spondylitis, Reiter’s disease, reactive arthritis) and is, therefore, used as part of the diagnostic procedure.

A positive HLA-B*27 result is associated with a high disease risk. In suspected cases of Ankylosing Spondylitis, a HLA-B*27 diagnosis provides an important contribution to the therapy of the patient. Around 3% to 6% of the people carrying the HLA-B*27 gene develop Ankylosing Spondylitis and more than 90% of all patients with a seronegative arthritis are carrying this gene. The HISTO TYPE B*27 Q kit covers all common HLA-B*27 subtypes. Moreover, the kit differentiates between the disease associated alleles and the subtypes HLA-B*27:06 or HLAB*27:09, which are not associated with Ankylosing Spondylitis

 

Test principle

Genomic DNA is amplified in a PCR with sequence-specific primers (SSP). Amplicons are detected with gene locus specific fluorescent dye-labelled hydrolysis probes (TaqMan® probes), which increases the diagnostic sensitivity and specificity of the test compared to a conventional SSP. If amplicons are present, the probes are hydrolyzed and a fluorescence signal is generated which increases proportionally to the amount of the PCR product. The fluorescence signals are measured by the optical detection unit of the RT-PCR-Cycler. The test is performed in a single PCR reaction that detects the internal positive control (human HBB gene) the disease-associated subtypes and the non-disease-associated subtypes with different fluorescent colours.


  •  • Discrimination of the non-associated alleles (B*27:06 and B*27:09 for Morbus Bechterew/Ankylosing Spondylitis).
  •  • Includes all common and well documented B*27 alleles covered in one PCR mix.
  •  • Internal amplification control included.
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    Instructions for Use- 

    HISTO TYPE B*27 Q Tests 
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