FastQ® Direct Real-time PCR
Technical information
Blood Booster for reproducible results directly from blood
- The FastQ® Direct product line incorporates a Blood Booster Reagent which ensure reliable and highly reproducible results even with difficult blood samples (e.g high EDTA concentration)
- Blood samples are simply diluted in the buffer and pipetted directly.
- Saves time, saves money & and reduces risk of cross contamination
- For performance date click here
Test Principle
- Ready-to-use reagents and the real-time PCR method provide a simple solution.
- Amplification from DNA sample with a wide range of concentrations
- The PCR run takes 90mins
- The PCR kit uses different fluorophores that are compatible with most common real-time cyclers. We have performed tests on the following devices:
- QuantStudio 6 (Thermo Fischer Scientific)
- CFX 96TM (Bio-Rad)
- Lightcycler® 480 (Roche)
- Analysis is performed using the software of the instrument manufacturer.
Product Description
FastQ B*27 direct detects the HLA-B*27 allele group associated with several different autoimmune diseases (Morbus Bechterew or Ankylosing Spondylitis, Reiter’s disease, reactive arthritis) and is, therefore, used as part of the diagnostic process.
- All CWD B*27 alleles are detected in a single PCR mix
- Internal amplification control included
Instructions for Use
FastQ B*27 direct
Product Description
FastQ CD direct Coming Soon
- Detects the relevant DQ alleles associated with coeliac disease in only two PCR mixes using four colours.
- See table below for alleles detected
- Internal amplification control
Product Description
FastQ HFE direct Coming Soon
- Multiplex Real-time PCR for hereditary haemochromatosis
- Detects the mutations H63D and C282Y for the HFE Gene
- Internal amplification control
To discuss your purchase requirements in complete confidence, please Contact us using our secure enquiry form, and one of our management team will get back in touch.
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