HISTO TYPE B*27 Real-time PCR

Technical information


Product Description


The HISTO TYPE B*27 quantitative real-time PCR kit is used for the molecular genetic detection of HLA-B*27 alleles. The HLA-B*27 protein is a variant of the human leucocyte antigen-B (HLA-B) and is associated with different autoimmune diseases (Morbus Bechterew or Ankylosing Spondylitis, Reiter’s disease, reactive arthritis) and is, therefore, used as part of the diagnostic procedure.


A positive HLA-B*27 result is associated with a high disease risk. In suspected cases of Ankylosing Spondylitis, a HLA-B*27 diagnosis provides an important contribution to the therapy of the patient. Around 3% to 6% of the people carrying the HLA-B*27 gene develop Ankylosing Spondylitis and more than 90% of all patients with a seronegative arthritis are carrying this gene. The HISTO TYPE B*27 Q kit covers all common HLA-B*27 subtypes. Moreover, the kit differentiates between the disease associated alleles and the subtypes HLA-B*27:06 or HLAB*27:09, which are not associated with Ankylosing Spondylitis.


Test Principle


Genomic DNA is amplified in a PCR with sequence-specific primers (SSP). Amplicons are detected with gene locus specific fluorescent dye-labelled hydrolysis probes (TaqMan® probes), which increases the diagnostic sensitivity and specificity of the test compared to a conventional SSP. If amplicons are present, the probes are hydrolyzed and a fluorescence signal is generated which increases proportionally to the amount of the PCR product. The fluorescence signals are measured by the optical detection unit of the RT-PCR-Cycler. The test is performed in a single PCR reaction that detects the internal positive control (human HBB gene) the disease-associated subtypes and the non-disease-associated subtypes with different fluorescent colours.
 
• Discrimination of the non-associated alleles (B*27:06 and B*27:09 for Morbus Bechterew/Ankylosing Spondylitis).

 • Includes all common and well documented B*27 alleles covered in one PCR mix.

 • Internal amplification control included.


Instructions for Use-
HISTO TYPE B*27 Q Tests


To discuss your purchase requirements in complete confidence, please Contact us using our secure enquiry form, and one of our management team will get back in touch.


Click any of the following links for further product information:


Share by: